By Richard M. Epand
The main lipid parts of organic membranes can endure many various and engaging morphological rearrangements. experiences of those different stages and the way during which they're shaped has a tendency to change the homes of standard bilayer membranes. This e-book examines the structural and organic roles of lipids forming non-lamellar buildings. Key beneficial properties * Characterization of non-lamellar constructions * Protein job and membrane homes * research of membrane fusion * impact of non-lamellar forming lipids on organic platforms
By Barry W. Hicks (Editor)
Barry W. Hicks and a crew of well-practiced experimentalists reveal the ability and the flexibility of the fairway fluorescent protein (GFP) marker with a state-of-the-art number of with ease reproducible GFP options. those comprise equipment for detecting and imaging GFP (FRET, BRET, and whole-body imaging); for utilizing GFP to create and choose for transgenic organisms, for its use as a biosensor; and for learning viruses. A invaluable significant other CD-ROM screens many colour figures and movies from over part the chapters.
By Christopher Jones, Barbara Mulloy, Adrian H. Thomas
This extraordinary survey of the potential purposes of physiochemical suggestions to the research of organic macromolecules is designed for the practising biochemist or biologist who desires to use the process yet lacks the event. The members emphasize useful points, comparable to constraints on pattern volume, purity, and presentation; the issues of time and cost concerned; the issues a strategy is most suitable to unravel; and the way the implications will be interpreted. one of the equipment tested are optical and infrared spectroscopy, microscopy, ultracentrifugation, calorimetry, X-ray and neutron scattering, and lightweight scattering. This ebook will permit the reader to with a bit of luck collaborate with experts in employing those innovations.
By Rune, Ed. Matthiesen
This can be an in-depth consultant to the speculation and perform of reading uncooked mass spectrometry (MS) info in proteomics. the quantity outlines to be had bioinformatics courses, algorithms, and databases on hand for MS information research. normal instructions for facts research utilizing se's equivalent to Mascot, Xtandem, and VEMS are supplied, with particular consciousness to making a choice on negative caliber facts and optimizing seek parameters.
By John A. Double, Michael J. Thompson
John A. Double and Michael J. Thompson have accumulated a severely very important sequence of novel and crucial ideas for learning telomeres and telomerase. those with no trouble reproducible tools offer state of the art instruments to spot, degree, and learn telomeres, to figure out telomerase expression on the RNA point, to figure out telomerase task, and to observe power modifiers of this job. The thoughts for assaying telomerase task diversity from regular radiological seize assays to nonradioactive tools, from non PCR-based how you can options utilizing real-time PCR. Telomeres and Telomerase: tools and Protocols presents the middle array of effective thoughts wanted at the present time to improve telomerase inhibitors or diagnostic/prognostic telomerase markers.
By Lucy G. Andrews, Trygve O. Tollefsbol (auth.), Lucy G. Andrews, Trygve O. Tollefsbol (eds.)
Due partially to the selective nature of telomerase inhibition as an anticancer technique, the sector has extended significantly some time past decade. the hot advances in tools of telomerase inhibition surround many various components of analysis together with molecular biology, telephone biology, biochemistry, oncology and gerontology. Telomerase Inhibition presents equipment and protocols for these researchers. The suggestions defined during this publication may still give you the researcher with a various and accomplished set of instruments with which to check telomerase inhibition. Leaders within the box offer lately built tools that experience frequent program similar to concentrating on the telomerase holoenzyme, its RNA template and different components linked to telomerase job. extra equipment concerning the screening of telomerase inhibitors and telomerase inhibition mixed with different chemotherapeutic brokers are awarded. this article, at the leading edge of the sphere, will offer investigators with the newest equipment utilized to the increasing box of telomerase inhibition.
By Joseph M. Chalovich (auth.), Ann O. Sperry (eds.)
Molecular motor proteins include 3 protein superfamilies: kinesins, dyneins and myosins. jointly, those proteins produce strength for stream in a really large choice of mobile tactics together with vesicular delivery, cellphone department, nuclear migration, muscle contraction, mechanochemical transduction, between others. additionally, molecular automobiles are expressed in either easy eukaryotes and in a tissue particular demeanour in larger cells. This tools quantity explores the intense sensible and structural range of molecular automobiles and provides tools proper to every motor kinfolk. moreover, it describes strategies directed at automobiles that fall outdoors of the three characterised households (i.e. dynamin and F1ATPase). This quantity of the Methods in Molecular Biology sequence is a necessary reference for telephone biologists studying the functionality of motor proteins in a variety of version organisms utilizing either in vitro and in vivo concepts. This quantity is a valuble resource for biochemists and molecular biologists as well.